{"id":8664,"date":"2023-05-25T07:28:41","date_gmt":"2023-05-25T07:28:41","guid":{"rendered":"https:\/\/tmckolkata.com\/in\/products-and-services-transfusion-medicine-copy\/"},"modified":"2025-12-26T06:48:45","modified_gmt":"2025-12-26T06:48:45","slug":"genotyping-transfusion-medicine","status":"publish","type":"page","link":"https:\/\/tmckolkata.com\/in\/genotyping-transfusion-medicine\/","title":{"rendered":"Blood group genotyping &#8211; Transfusion Medicine"},"content":{"rendered":"<div id='av-layout-grid-1'  class='av-layout-grid-container av-2ymm6y-4807741fc6365891d0b9190d6c51310e entry-content-wrapper main_color av-flex-cells  avia-builder-el-0  el_before_av_layout_row  avia-builder-el-first   container_wrap sidebar_right'  >\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-kluustas-59701dfd68b8cc08d09d999709952f42\">\n.flex_cell.av-kluustas-59701dfd68b8cc08d09d999709952f42{\nvertical-align:middle;\nbackground:url(https:\/\/tmckolkata.com\/in\/wp-content\/uploads\/2021\/03\/BloodBan_header1.jpg) 50% 50% no-repeat scroll ;\n}\n.responsive #top #wrap_all .flex_cell.av-kluustas-59701dfd68b8cc08d09d999709952f42{\npadding:100px 100px 100px 100px !important;\n}\n<\/style>\n<div class='flex_cell av-kluustas-59701dfd68b8cc08d09d999709952f42 av-gridrow-cell av_one_full no_margin  avia-builder-el-1  avia-builder-el-no-sibling  content-align-middle avia-full-stretch'  ><div class='flex_cell_inner'><\/div><\/div>\n<\/div>\n<div id='av-layout-grid-2'  class='av-layout-grid-container av-bjulrn-dfd66c264cccc35771e30c0512eacf8c entry-content-wrapper main_color av-flex-cells  avia-builder-el-2  el_after_av_layout_row  el_before_av_heading  grid-row-not-first  container_wrap sidebar_right'  >\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lhhdffwk-b2b9fc91b5b37221f75e217eee7c33fc\">\n.flex_cell.av-lhhdffwk-b2b9fc91b5b37221f75e217eee7c33fc{\nvertical-align:middle;\nbackground-color:#2388c2;\n}\n.responsive #top #wrap_all .flex_cell.av-lhhdffwk-b2b9fc91b5b37221f75e217eee7c33fc{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-lhhdffwk-b2b9fc91b5b37221f75e217eee7c33fc av-gridrow-cell av_one_fourth no_margin  avia-builder-el-3  el_before_av_cell_one_fourth  avia-builder-el-first  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lhhdg5z8-25092833b8cdcaf731f5247599546442\">\n.flex_cell.av-lhhdg5z8-25092833b8cdcaf731f5247599546442{\nvertical-align:middle;\nbackground-color:#379cd6;\n}\n.responsive #top #wrap_all .flex_cell.av-lhhdg5z8-25092833b8cdcaf731f5247599546442{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-lhhdg5z8-25092833b8cdcaf731f5247599546442 av-gridrow-cell av_one_fourth no_margin  avia-builder-el-4  el_after_av_cell_one_fourth  el_before_av_cell_one_fourth  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lhhdgolw-baa949d6d8942bbf7afaa4b585789639\">\n.flex_cell.av-lhhdgolw-baa949d6d8942bbf7afaa4b585789639{\nvertical-align:middle;\nbackground-color:#5fb4e4;\n}\n.responsive #top #wrap_all .flex_cell.av-lhhdgolw-baa949d6d8942bbf7afaa4b585789639{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-lhhdgolw-baa949d6d8942bbf7afaa4b585789639 av-gridrow-cell av_one_fourth no_margin  avia-builder-el-5  el_after_av_cell_one_fourth  el_before_av_cell_one_fourth  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lhhdh844-cd790256d030facbd84bf50cf5002708\">\n.flex_cell.av-lhhdh844-cd790256d030facbd84bf50cf5002708{\nvertical-align:middle;\nbackground-color:#80c7ef;\n}\n.responsive #top #wrap_all .flex_cell.av-lhhdh844-cd790256d030facbd84bf50cf5002708{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-lhhdh844-cd790256d030facbd84bf50cf5002708 av-gridrow-cell av_one_fourth no_margin  avia-builder-el-6  el_after_av_cell_one_fourth  avia-builder-el-last  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<\/div><div id='after_grid_row_2'  class='main_color av_default_container_wrap container_wrap sidebar_right'  ><div class='container av-section-cont-open' ><div class='template-page content  av-content-small alpha units'><div class='post-entry post-entry-type-page post-entry-8664'><div class='entry-content-wrapper clearfix'>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-klutz8ls-e57e3eef67492e2340b3f650cc62c695\">\n#top .av-special-heading.av-klutz8ls-e57e3eef67492e2340b3f650cc62c695{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-klutz8ls-e57e3eef67492e2340b3f650cc62c695 .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-klutz8ls-e57e3eef67492e2340b3f650cc62c695 .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-klutz8ls-e57e3eef67492e2340b3f650cc62c695 av-special-heading-h3 blockquote elegant-quote elegant-centered  avia-builder-el-7  el_after_av_layout_row  el_before_av_hr  avia-builder-el-first '><h3 class='av-special-heading-tag '  itemprop=\"headline\"  ><span class=\"heading-wrap\">Blood Group Genotyping<\/span><\/h3><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lhio9cur-d4610d6b3b7888586bb02876da7b80b4\">\n#top .hr.hr-invisible.av-lhio9cur-d4610d6b3b7888586bb02876da7b80b4{\nheight:20px;\n}\n<\/style>\n<div  class='hr av-lhio9cur-d4610d6b3b7888586bb02876da7b80b4 hr-invisible  avia-builder-el-8  el_after_av_heading  el_before_av_one_half '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<div  class='flex_column av-3x4op6-a472816fee720a7456c414935603ac9b av_one_half  avia-builder-el-9  el_after_av_hr  el_before_av_one_half  first flex_column_div  '     ><section  class='av_textblock_section av-kl96eiv0-6d2d3b8215213988d4e00e381e2ddbbd '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h5><strong>Sample requirements:<\/strong><\/h5>\n<p style=\"text-align: justify;\">At least 6 mL of EDTA blood sample is required (two samples, 6 mL each would be preferred). EDTA samples should be sent in coolant gel pack to maintain the temperature around 2-8\u1d3cC along with the properly filled up case record form. The blood samples should be reached to the TMC blood centre within 48 hours of collection.<\/p>\n<h6><strong>Blood group antigens that are being determined by genotyping:<\/strong><\/h6>\n<ul>\n<li>Rh D, Rh C, Rh c, Rh E, Rh e, Rh C<sup>w<\/sup>,<\/li>\n<li>KEL1 (Kell), KEL2 (Cellano),<\/li>\n<li>Jk<sup>a <\/sup>(Kidd a), Jk<sup>b <\/sup>(Kidd b),<\/li>\n<li>Fy<sup> a<\/sup> (Duffy a), Fy<sup>b <\/sup>(Duffy b),<\/li>\n<li>M, N, S, small s,<\/li>\n<li>Do a (Dombrock a) and Do b (Dombrock b),<\/li>\n<li>Vel+\/Vel-<\/li>\n<\/ul>\n<\/div><\/section><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-mjmibbw2-f1d1e2e28eb2de45b138c00c8a2d02f9\">\n@keyframes av_boxShadowEffect_av-mjmibbw2-f1d1e2e28eb2de45b138c00c8a2d02f9 {\n0%   { box-shadow:  0 0 0 0 ; opacity: 1; }\n100% { box-shadow:  0 0 10px 0 ; opacity: 1; }\n}\n.avia-image-container.av-mjmibbw2-f1d1e2e28eb2de45b138c00c8a2d02f9 img.avia_image{\nbox-shadow: 0 0 10px 0 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srcset=\"https:\/\/tmckolkata.com\/in\/wp-content\/uploads\/2025\/12\/Tranfusion-Medinine-Research-dashboard-2025.jpg 960w, https:\/\/tmckolkata.com\/in\/wp-content\/uploads\/2025\/12\/Tranfusion-Medinine-Research-dashboard-2025-768x432.jpg 768w, https:\/\/tmckolkata.com\/in\/wp-content\/uploads\/2025\/12\/Tranfusion-Medinine-Research-dashboard-2025-705x397.jpg 705w\" sizes=\"(max-width: 960px) 100vw, 960px\" \/><\/div><\/div><\/div><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-mjmifbh2-b44fe012492a649e6e69981b9ee5fb5a\">\n#top .hr.hr-invisible.av-mjmifbh2-b44fe012492a649e6e69981b9ee5fb5a{\nheight:20px;\n}\n<\/style>\n<div  class='hr av-mjmifbh2-b44fe012492a649e6e69981b9ee5fb5a hr-invisible  avia-builder-el-12  el_after_av_image  avia-builder-el-last '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><\/p><\/div><div  class='flex_column av-2behvu-0964bfbc4ed132bce10c6272cd02cb18 av_one_half  avia-builder-el-13  el_after_av_one_half  el_before_av_heading  flex_column_div  '     ><style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lhio75sp-d25f0bb10b620628a2436eff49ab7513\">\n.av_promobox.av-lhio75sp-d25f0bb10b620628a2436eff49ab7513{\ncolor:#ffffff;\nbackground-color:#444444;\nborder-style:groove;\nborder-radius:5px 5px 5px 5px;\nbox-shadow: 5px 5px 2px 2px #e0e0e0;\n}\n<\/style>\n<div  class='av_promobox av-lhio75sp-d25f0bb10b620628a2436eff49ab7513 avia-button-no  avia-builder-el-14  el_before_av_hr  avia-builder-el-first '><div class='avia-promocontent'><p style=\"text-align: center;\"><strong>Contact for payment details: <\/strong>033-66057603<\/p>\n<p style=\"text-align: center;\"><strong>Sample receiving time: <\/strong>Monday to Friday: 9am to 5 pm<\/p>\n<p style=\"text-align: center;\"><strong>Reporting time: <\/strong>After 7days of receiving the sample at TMC blood centre<\/p>\n<p>\n<\/div><\/div><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-li2whrzo-1ebad51e876955228bcee7a558ed34c8\">\n#top .hr.hr-invisible.av-li2whrzo-1ebad51e876955228bcee7a558ed34c8{\nheight:15px;\n}\n<\/style>\n<div  class='hr av-li2whrzo-1ebad51e876955228bcee7a558ed34c8 hr-invisible  avia-builder-el-15  el_after_av_promobox  el_before_av_textblock '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><br \/>\n<section  class='av_textblock_section av-lj8fnvsm-571c017058dcf7ba2c78fd776762e638 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><h6 style=\"text-align: center;\"><strong>Instruction for sending samples<\/strong><\/h6>\n<ol>\n<li>Send the sample along with the <em>hard copy<\/em> of <em>Case Record Form, Medical Registration Form <\/em>and a <em>copy of self-attested Aadhar Card<\/em> to the shipping address.<\/li>\n<li>Send the <em>soft copy<\/em> of the above mentioned documents to this email id: <em><strong>blood-bank@tmckolkata.com<\/strong><\/em><\/li>\n<\/ol>\n<\/div><\/section><br \/>\n<div  class='avia-button-wrap avia-button-center  avia-builder-el-17  el_after_av_textblock  el_before_av_hr '>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-li2w9b25-d8bfc12d8bff86b83f569cca51c55753\">\n#top #wrap_all .avia-button.av-li2w9b25-d8bfc12d8bff86b83f569cca51c55753{\nborder-radius:5px 5px 5px 5px;\nbox-shadow: 5px 5px 2px 2px #e0e0e0;\ntransition:all 0.4s ease-in-out;\nfont-size:18px;\n}\n#top #wrap_all .avia-button.av-li2w9b25-d8bfc12d8bff86b83f569cca51c55753:hover{\nbackground-color:#8e0000;\ncolor:white;\ntransition:all 0.4s ease-in-out;\n}\n#top #wrap_all .avia-button.av-li2w9b25-d8bfc12d8bff86b83f569cca51c55753:hover .avia_button_background{\nborder-radius:5px 5px 5px 5px;\n}\n#top #wrap_all .avia-button.av-li2w9b25-d8bfc12d8bff86b83f569cca51c55753:hover .avia-svg-icon svg:first-child{\nfill:white;\nstroke:white;\n}\n<\/style>\n<a href='https:\/\/tmckolkata.com\/services\/Case_record_form_for_blood_group_genotyping_reg.pdf' class='avia-color-red avia-button avia-button-fullwidth av-li2w9b25-d8bfc12d8bff86b83f569cca51c55753 avia-icon_select-yes-left-icon avia-color-red'   aria-label=\"Download case record form &amp; Registration form\"><span class='avia_button_icon avia_button_icon_left avia-iconfont avia-font-entypo-fontello' data-av_icon='\ue86f' data-av_iconfont='entypo-fontello' ><\/span><span class='avia_iconbox_title' >Download case record form &amp; Registration form<\/span><\/a><\/div><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lie6l699-38ad5f7868fc1564f239faab75ef47f5\">\n#top .hr.hr-invisible.av-lie6l699-38ad5f7868fc1564f239faab75ef47f5{\nheight:15px;\n}\n<\/style>\n<div  class='hr av-lie6l699-38ad5f7868fc1564f239faab75ef47f5 hr-invisible  avia-builder-el-18  el_after_av_button_big  el_before_av_promobox  avia-builder-el-first '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div><br \/>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lie6jphu-c5495e915526b91f4bebb306fd5e81da\">\n.av_promobox.av-lie6jphu-c5495e915526b91f4bebb306fd5e81da{\ncolor:#ffffff;\nbackground-color:#444444;\n}\n<\/style>\n<div  class='av_promobox av-lie6jphu-c5495e915526b91f4bebb306fd5e81da avia-button-yes  avia-builder-el-19  el_after_av_hr  avia-builder-el-last '><div class='avia-promocontent'><p>\n<strong>Research and Publications<\/strong><\/p>\n<\/div><div  class='avia-button-wrap av-lie6jphu-a2090bce148a1c9cb9f22f771e5eff0b-wrap avia-button-right '><a href='https:\/\/tmckolkata.com\/in\/professionals\/research-and-publications\/#transfusion-medicine'  class='avia-button av-lie6jphu-a2090bce148a1c9cb9f22f771e5eff0b av-link-btn avia-icon_select-yes-left-icon avia-size-large avia-position-right avia-color-theme-color'   aria-label=\"Click to know more\"><span class='avia_button_icon avia_button_icon_left avia-iconfont avia-font-entypo-fontello' data-av_icon='\ue875' data-av_iconfont='entypo-fontello' ><\/span><span class='avia_iconbox_title' >Click to know more<\/span><\/a><\/div><\/div><\/p><\/div><\/p>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-li2w3lcd-89dfa05f844f8f4ec472a467b0fd02cc\">\n#top .av-special-heading.av-li2w3lcd-89dfa05f844f8f4ec472a467b0fd02cc{\npadding-bottom:10px;\n}\nbody .av-special-heading.av-li2w3lcd-89dfa05f844f8f4ec472a467b0fd02cc .av-special-heading-tag .heading-char{\nfont-size:25px;\n}\n.av-special-heading.av-li2w3lcd-89dfa05f844f8f4ec472a467b0fd02cc .av-subheading{\nfont-size:15px;\n}\n<\/style>\n<div  class='av-special-heading av-li2w3lcd-89dfa05f844f8f4ec472a467b0fd02cc av-special-heading-h5  avia-builder-el-20  el_after_av_one_half  el_before_av_textblock  av-thin-font'><h5 class='av-special-heading-tag '  itemprop=\"headline\"  ><strong>FAQs about red cell genotyping<\/strong><\/h5><div class=\"special-heading-border\"><div class=\"special-heading-inner-border\"><\/div><\/div><\/div>\n<section  class='av_textblock_section av-lhiod5zb-75e844af35e40bb9e3f6a6b010d108a0 '   itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock'  itemprop=\"text\" ><p><strong>Q1. What is blood group genotyping?<\/strong><\/p>\n<p style=\"text-align: justify;\">It is a molecular method of blood grouping where DNA is extracted from blood (white blood cells) and amplified by using sequence specific primers to predict certain blood group antigens present on the red blood cell membrane. It is based on the PCR technique. The final amplified DNA segments are separated by gel electrophoresis and then the final report is generated.<\/p>\n<p><strong>Q2. How is it different from red cell phenotyping?<\/strong><\/p>\n<p style=\"text-align: justify;\">Yes, it is different. The red cell phenotyping is based on the hemagglutination principle to detect certain blood group antigens on the red blood cells. On the contrary, red cell genotyping is a DNA based technique.<\/p>\n<p><strong>Q3. Is it done in the donor or patient?<\/strong><\/p>\n<p style=\"text-align: justify;\">It can be done both in donors or patients. Here at TMC blood centre tests are predominantly performed in patients.<\/p>\n<p><strong>Q4. In which group of patients the genotyping is performed?<img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-8710\" src=\"https:\/\/tmckolkata.com\/in\/wp-content\/uploads\/2023\/05\/Bloodbank_10.jpg\" alt=\"\" width=\"480\" height=\"270\" \/><\/strong><\/p>\n<ul>\n<li>Patients who have been recently transfused (last 3 months)<\/li>\n<li>Patients who are DAT positive (can\u2019t be phenotyped)<\/li>\n<li>\u00a0In alloantibody identification where serology is inconclusive<\/li>\n<li>Patient who are on anti-CD38 or anti-CD47 drugs<\/li>\n<\/ul>\n<p><strong>Q5. What are the benefits to these patients?<\/strong><\/p>\n<p style=\"text-align: justify;\">By genotyping the phenotype (most of the clinically significant red cell antigens) can be predicted in the recently transfused or AIHA patients. By transfusing phenotype matched red cells to these patients, development of further alloantibodies could be prevented and transfusion management can be optimized.<\/p>\n<\/div><\/section>\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-lhk41dwe-262f1fdfd40c9af98c37984de8c51776\">\n#top .hr.hr-invisible.av-lhk41dwe-262f1fdfd40c9af98c37984de8c51776{\nmargin-top:-30px;\nheight:1px;\n}\n<\/style>\n<div  class='hr av-lhk41dwe-262f1fdfd40c9af98c37984de8c51776 hr-invisible  avia-builder-el-22  el_after_av_textblock  el_before_av_layout_row  avia-builder-el-last '><span class='hr-inner '><span class=\"hr-inner-style\"><\/span><\/span><\/div>\n<\/div><\/div><\/div><!-- close content main div --><\/div><\/div><div id='av-layout-grid-3'  class='av-layout-grid-container av-999cqb-48e50a2329b7d6a08a677bc54b35d6b1 entry-content-wrapper main_color av-flex-cells  avia-builder-el-23  el_after_av_hr  avia-builder-el-last  grid-row-not-first  container_wrap sidebar_right'  >\n\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-7qcbw3-725cb733f3ce7e65750ccbcdee450c4d\">\n.flex_cell.av-7qcbw3-725cb733f3ce7e65750ccbcdee450c4d{\nvertical-align:middle;\nbackground-color:#2388c2;\n}\n.responsive #top #wrap_all .flex_cell.av-7qcbw3-725cb733f3ce7e65750ccbcdee450c4d{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-7qcbw3-725cb733f3ce7e65750ccbcdee450c4d av-gridrow-cell av_one_fourth no_margin  avia-builder-el-24  el_before_av_cell_one_fourth  avia-builder-el-first  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-6enic3-89ef524c41369659d0274ba3987d453f\">\n.flex_cell.av-6enic3-89ef524c41369659d0274ba3987d453f{\nvertical-align:middle;\nbackground-color:#379cd6;\n}\n.responsive #top #wrap_all .flex_cell.av-6enic3-89ef524c41369659d0274ba3987d453f{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-6enic3-89ef524c41369659d0274ba3987d453f av-gridrow-cell av_one_fourth no_margin  avia-builder-el-25  el_after_av_cell_one_fourth  el_before_av_cell_one_fourth  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-1c6z7-43fa9a90f2878f0db8da706d8361e445\">\n.flex_cell.av-1c6z7-43fa9a90f2878f0db8da706d8361e445{\nvertical-align:middle;\nbackground-color:#5fb4e4;\n}\n.responsive #top #wrap_all .flex_cell.av-1c6z7-43fa9a90f2878f0db8da706d8361e445{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-1c6z7-43fa9a90f2878f0db8da706d8361e445 av-gridrow-cell av_one_fourth no_margin  avia-builder-el-26  el_after_av_cell_one_fourth  el_before_av_cell_one_fourth  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<style type=\"text\/css\" data-created_by=\"avia_inline_auto\" id=\"style-css-av-7xw2r-9fa0b6c25e837421d10551bdaddee2fd\">\n.flex_cell.av-7xw2r-9fa0b6c25e837421d10551bdaddee2fd{\nvertical-align:middle;\nbackground-color:#80c7ef;\n}\n.responsive #top #wrap_all .flex_cell.av-7xw2r-9fa0b6c25e837421d10551bdaddee2fd{\npadding:15px 15px 15px 15px !important;\n}\n<\/style>\n<div class='flex_cell av-7xw2r-9fa0b6c25e837421d10551bdaddee2fd av-gridrow-cell av_one_fourth no_margin  avia-builder-el-27  el_after_av_cell_one_fourth  avia-builder-el-last  content-align-middle'  ><div class='flex_cell_inner'><\/div><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-8664","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/pages\/8664","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/comments?post=8664"}],"version-history":[{"count":2,"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/pages\/8664\/revisions"}],"predecessor-version":[{"id":11363,"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/pages\/8664\/revisions\/11363"}],"wp:attachment":[{"href":"https:\/\/tmckolkata.com\/in\/wp-json\/wp\/v2\/media?parent=8664"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}